Abstract

Methylglyoxal is a reactive α, β dicarbonyl aldehyde compound that originates from various biochemical pathways. Some studies suggest that increased methylglyoxal in blood leads to changes in fibrinolysis; however, the precise mechanism is not clear. The aim of this study was to compare different concentrations of methylglyoxal and aspirin on fibrinolysis in the plasma of healthy individuals in vitro. Different concentrations of methylglyoxal (5, 50, 100, and 500μmol/l) and aspirin (1, 10, and 100mg/l) were added to the plasma citrate. They were incubated at 37 °C for 24h. Then, fibrinolysis parameters were analyzed by the turbidimetric procedure at 405nm. The Independent Samples t-test was utilized to compare them (P<0.05). Findings revealed that methylglyoxal at 500μmol/l with aspirin 100mg/l had significant changes in the maximum lysis velocity (0.163±0.003), half-time lysis (240±10.00), the total lysis time (485±5.00), lag time in lysis (126±5.77), compared with methylglyoxal at 500μmol/l (0.104±0.005), (276±5.77), (570±10.00), and (186±5.77), respectively (P<0.05). Methylglyoxal at 500μmol/l with aspirin 1mg/l did not significantly change in either parameter (P>0.05). Methylglyoxal at 100μmol/l with aspirin 1mg/l did not significantly change in either fibrinolysis parameter (P>0.05), compared with methylglyoxal at 100μmol/l. Methylglyoxal at 5μmol/l with aspirin (1, 10, 100mg/l) changed in all fibrinolysis parameters (P<0.05), compared with methylglyoxal at 5μmol/l. The other concentrations were compared in the same way. Aspirin (more than 1mg/l) had more effect on higher concentrations of methylglyoxal. It increased the velocity of lysis of the clot and shortened clot lysis. © 2013 Wolters Kluwer Health | Lippincott Williams & Wilkins.