(2024) Evaluation of the effect of mitoquinone on functional parameters, DNA structure, and genes expression related to the apoptotic and antioxidants of human sperm after freezing–thawing. Molecular Biology Reports. ISSN 03014851 (ISSN)
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Abstract
Objective: Sperm freezing is considered as an effective way in assisted reproductive technology (ART) programs, it has detrimental effects on sperm function, due to the production of reactive oxygen species (ROS). This study aimed to investigate the potential of Mitoquinone (MitoQ) in inhibiting the production of mitochondrial ROS during sperm freezing. Methods: A total of 20 human normozoosperm samples were collected for this study. The samples were divided into four groups, each containing different concentrations of MitoQ (0, 0.2, 2, and 20 nM), and then subjected to the freezing process. After thawing, the sperm suspensions were evaluated for parameters including motility, morphology, acrosome integrity, adenosine triphosphate (ATP) level, intracellular ROS, viability, chromatin packaging, DNA denaturation, DNA fragmentation, as well as the expression of antioxidants (GPX, SOD) and apoptotic (Bax, Bcl2) genes. Results: The results showed that total and progressive mobility of sperms significantly increased in the 2 nM group, while significantly decreased in the 20 nM group (p ≤ 0.05). Sperm morphology did not significantly improve across all the tested concentrations (p ≥ 0.05). Intracellular ROS levels showed a significant decrease and increase in the concentrations of 2 and 20 nM, respectively (p ≤ 0.05). Furthermore, a significant increase was observed in viability, ATP, acrosome integrity, chromatin packaging, and non-denatured and non-fragmented DNA after treatment with 2 nM of MitoQ, compared with the control group (p ≤ 0.05). Regarding gene expressions, the relative expressions of oxidative stress genes were increased in the 2 nM group and decreased in the 20 nM group (p ≤ 0.05), while no significant difference was observed in the expressions of apoptotic genes compared with the control group (p ≥ 0.05). All the comparisons were made with respect to the control group. Conclusion: Adding the optimal concentration of MitoQ (2 nM) to the sperm freezing medium not only improves sperm functional parameters and reduces DNA damages, but also stimulates the expression of antioxidant genes, leading to even greater benefits for sperm cryopreservation. © 2024, The Author(s), under exclusive licence to Springer Nature B.V.
| Item Type: | Article |
|---|---|
| Keywords: | Chromatin packaging DNA fragmentation Gene expression Human sperm Adenosine Triphosphate Antioxidants Chromatin DNA Freezing Humans Male Organophosphorus Compounds Reactive Oxygen Species Semen Spermatozoa Ubiquinone antioxidant glutathione peroxidase mitoquinone protein Bax protein bcl 2 superoxide dismutase unclassified drug organophosphorus compound reactive oxygen metabolite acrosome antioxidant activity apoptosis Article cell suspension chromatin structure concentration response controlled study DNA denaturation DNA structure freeze thawing human human cell mitochondrion oxidative stress protein expression semen analysis semen cryopreservation semen parameters sperm function sperm morphology sperm viability spermatozoon motility sperm spermatozoon |
| Journal or Publication Title: | Molecular Biology Reports |
| Journal Index: | Scopus |
| Volume: | 51 |
| Number: | 1 |
| Identification Number: | https://doi.org/10.1007/s11033-023-09020-0 |
| ISSN: | 03014851 (ISSN) |
| Depositing User: | ms soheila Bazm |
| URI: | http://eprints.ssu.ac.ir/id/eprint/34110 |
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