Abstract

Background: Recurrent spontaneous abortion (RSA), defined as two or more consecutive pregnancy losses before the 20th week of gestation. Foxp3 is a regulatory T-cell-dependent transcription factor that is essential for function of these cells. The aim of this study was to identify the polymorphism Foxp3 (-3279) gene in the women with recurrent abortion and controls. Methods: In this case-control study, 80 women with history of at least two spontaneous abortion before the 20th week of gestation determined as case; they were negative for genetic, infectious, anatomic and hormonal disorders. Eighty non-pregnant healthy women with no history of abortion and having at least one child that referred to Yazd centeral laboratory for check-up were selected as control. The Foxp3 gene 3279 polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was performed. Data analysis was performed by chi-square. Findings: The frequency of CC, AC and AA genotypes were 58.8, 25, and 16.2 percent in women with RSA and 52.5, 21.2 and 26.2 percent in controls, respectively. No significant difference was found between the groups (P = 0.3). Conclusion: In this study, a statistically significant difference was not seen in the prevalence of polymorphisms rs3761548C/A between control and recurrent abortion groups.