(2015) Melatonin attenuates lung injury in a hind limb ischemia-reperfusion rat model. Revista Portuguesa de Pneumologia. pp. 30-35. ISSN 08732159 (ISSN)
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Abstract
Objective: This study evaluated the protective antioxidant effect of melatonin on lung injury as a remote organ after skeletal muscle ischemia-reperfusion in rats. Methods: Thirty male Wistar rats were allocated randomly into three experimental groups: operated with no ischemia (Sham) group, ischemia-reperfusion group and ischemia-reperfusion + melatonin group. Hind limb ischemia was induced by clamping the femoral artery. After 2 h ischemia, the clamp was removed and the animal underwent 24 h reperfusion. Rats in the ischemia-reperfusion + melatonin group received melatonin (10 mg/kg i.v.), immediately before the clamp was removed. At the end of the trial, animals were euthanized and the lungs were removed for water content determination, histopathological and biochemical studies. Results: In the ischemia-reperfusion + melatonin group, tissues showed less intense histological abnormalities such as neutrophilic infiltration, intra-alveolar hemorrhage and edema compared with the ischemia-reperfusion group. Histopathologically, there was a significant difference (P < 0.05) between the two groups. The lung water content in the ischemia-reperfusion + melatonin group was significantly lower than the ischemia-reperfusion group (P < 0.05). Lung tissue myeloperoxidase (MPO) activity and nitric oxide (NO) level were significantly (P < 0.05) increased by ischemia-reperfusion. The increase in these parameters was reduced by melatonin. Comparing the ischemia-reperfusion + melatonin group with the sham group, no significant increase in all analyzed aspects of the research was observed. © 2013 Sociedade Portuguesa de Pneumologia.
| Item Type: | Article |
|---|---|
| Keywords: | Histopathology Ischemia-reperfusion Lung remote injury Melatonin Myeloperoxidase Nitric oxide Acute Lung Injury Animals Antioxidants Disease Models, Animal Lower Extremity Male Muscle, Skeletal Random Allocation Rats Rats, Wistar Reperfusion Injury antioxidant animal experiment animal model animal tissue antioxidant activity artery occlusion Article controlled study enzyme activity femoral artery hindlimb limb ischemia lung edema lung fluid lung hemorrhage lung weight neutrophil chemotaxis nonhuman rat animal complication disease model lower limb pathology randomization skeletal muscle vascularization Wistar rat |
| Page Range: | pp. 30-35 |
| Journal or Publication Title: | Revista Portuguesa de Pneumologia |
| Volume: | 21 |
| Number: | 1 |
| Identification Number: | https://doi.org/10.1016/j.rppnen.2014.01.010 |
| ISSN: | 08732159 (ISSN) |
| Depositing User: | Mr mahdi sharifi |
| URI: | http://eprints.ssu.ac.ir/id/eprint/32451 |
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