Abstract

Background and Aim: Today, research in the field of tissue engineering has increased widely. Scaffold production is important according to the morphology of the tissues. The purpose of producing this scaffold is to make a uterine endometrial scaffold,. So, the fibers with different diameters and morphology were synthesized to select the optimal scaffold. Materials and Methods: In this study, electrospun hybrid fiber scaffolds made of polycaprolactone (PCL), gelatin (G) and polydimethylsiloxane (PDMS) with different optimal ratios and different morphologies were produced by the electrospinning collector changing for tissue engineering applications. Physicochemical properties of fabricated scaffolds were evaluated using scanning electron microscopy, the immersion of scaffold samples, attenuated total reflectance Fourier transform infrared and tensile strength analysis. Cytotoxicity analyses of scaffolds and human foreskin fibroblasts on the scaffolds were assessed by 3-(4, 5-dimethylthiazoyl-2-yl) 2, 5-diphenyltetrazolium bromide assay. Attachment to the scaffolds and morphology of fibroblasts on them were evaluated by hematoxylin-eosin staining. Results: Layer-by-layer scaffold, cotton-like scaffold, mixed cell scaffold, flat scaffold and control sample were reduced in terms of cell growth and proliferation, respectively. Therefore, layer-by-layer scaffold and cotton-like scaffolds are the best options for cell growth and proliferation. Also, the mechanical properties of layer-by-layer scaffold and cotton-like scaffolds are better than other scaffolds. The porosity of cotton-like scaffold is better than other scaffolds and is suitable for cell penetration into the scaffold. Although layer-by-layer and cell-mixed scaffolds perform well due to the cell's location between the fibers. Conclusion: The morphology and characteristics of the PCL / G / PDMS hybrid scaffolds are adjustable by the electrospinning collector changing. The PCL/ G/ PDMS hybrid scaffolds characteristics show that these scaffolds are suitable for tissue engineering applications especially engineering of elastic tissues. © 2018 the Author (s).