(2023) The effects of purslane<i> (Portulaca</i><i> oleracea)</i> and fennel<i> (Foeniculum</i><i> vulgare</i><i> Mill)</i> hydroalcoholic extracts on the functional parameters of human spermatozoa after vitrification. Clinical and Experimental Reproductive Medicine-Cerm. pp. 78-85. ISSN 2233-8233
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Abstract
Objective: Reactive oxygen species (ROS) are produced during cryopreservation of human sperm and impair sperm function. Antioxidant compounds, such as fennel and purslane, reduce the damaging effects of ROS. This study aimed to evaluate motility parameters, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), intracellular ROS, and DNA damage to determine the optimum con-centrations of hydroalcoholic extracts of fennel and purslane for human spermatozoa cryopreservation. Methods: Twenty human sperm samples were used and divided into seven equal groups consisting of fennel hydroalcoholic extract (5, 10, and 15 mg/L), purslane hydroalcoholic extract (25, 50, and 100 mg/L), and no additive. Results: Supplementation of 25 mg/L and 50 mg/L purslane extract and 10 mg/L fennel extract in cryopreservation extender significantly increased the motility and PMI of sperm with a significant reduction in intracellular ROS compared to control groups (p<0.05). A 50 mg/L concentration of purslane extract elevated progressive motility and MMP compared to the control group (p<0.05). No significant differences were seen for motion patterns and DNA damage of frozen-thawed human sperm in extender containing these extracts. Conclusion: The results showed that supplementation of 50 mg/L purslane extract and 10 mg/L fennel extract in semen cryopreservation extender has the potential to decrease intracellular ROS and subsequently elevate the motility and PMI of human sperm.
Item Type: | Article |
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Keywords: | Fennel Human sperm Purslane Vitrification sperm l. antioxidants extenders toxicity cysteine fruit mice Obstetrics & Gynecology Reproductive Biology |
Page Range: | pp. 78-85 |
Journal or Publication Title: | Clinical and Experimental Reproductive Medicine-Cerm |
Journal Index: | WoS |
Volume: | 50 |
Number: | 2 |
Identification Number: | https://doi.org/10.5653/cerm.2021.04805 |
ISSN: | 2233-8233 |
Depositing User: | Mr mahdi sharifi |
URI: | http://eprints.ssu.ac.ir/id/eprint/29857 |
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