Abstract

In this study, the germ of Date seed was collected by breaking a thousand date kernels. These acquired germ was powdered and dissolved in distilled water for achievement of different concentrations (3.4, 1.7, 0.85, 0.425 and 0.212 mg /ml). Cancerous (MCF-7) and normal (HFF) cell lines were treated with these concentrations for 24, 48, and 72 hours. Cell viability was assessed using MTT technique. P53 gene expression was evaluated by Real time PCR technique. Results showed that in the highest concentration of germ of Date seed (3.4mg/ml), the percentage of viable cells for cancerous cell lines was 26, 30.1, 40.1 in 24, 48, and 72 hours, respectively. In this concentration, for normal cell lines, the percentage of viable cells was 36.1, 37.1, 42.1 in 24, 48, and 72 hours, respectively. In both cell line, germ of Date seed led to increase of P53 gene expression.