Abstract

We report a case of healthy live birth from a couple diagnosed with oligoasthenoteratozoospermia (OAT). They failed three ICSI cycles in the past 11 years. In the last cycle, the ovarian stimulation was done with antagonist protocol and six oocytes were aspirated. Semen sample was prepared by one layer gradient. 10 class I spermatozoa were selected by motile-sperm organelle-morphology examination (MSOME) according to Cassuto criteria. The selected spermatozoa were then injected into the MII oocytes. 16-18 h after ICSI, three zygotes was formed and, then, cultured in the time lapse monitoring (TLM) for 3 days. Two best embryos were selected according to the morphokinetic status for transfer. A singleton pregnancy was achieved, resulting in the birth of a healthy baby. This successful outcome shows that use of high technologies of MSOME and TLM procedures are useful for selection of the best spermatozoa and embryos in case of severe male infertility. (C) 2019 Elsevier Masson SAS. All rights reserved.