Abstract

convergence of nanotechnology, medicine, biology and chemistry has provided novel solutionsfor efficient diagnosis systems for disease markers. A detection method of troponin I (TnI), as the goldstandard of acute myocardial infarction biomarker, was investigated and a novel aptasensor was pre-sented for this biomarker. An array of gold nanodumbbells was firstly synthesized using putrescine asthe shape directing agent at a surface. It was then applied as a transducer for the immobilization of a76-mer TnI aptamer to fabricate a simple electrochemical TnI aptasensor. Using the aptasensor, TnI wasdetected in a linear range of 0.05–500 ng mL−1with a limit of detection of 8.0 pg mL−1. The aptasensorshowed a diagnostic sensitivity of 100 and a diagnostic specificity of 85 when challenged with bloodserum samples of 89 individuals.