Repository of Research and Investigative Information

Repository of Research and Investigative Information

Shahid Sadoughi University of Medical Sciences

Designing of a novel scaffold based on human serum albumin and hydroxyapatite nanoparticles, and the study of its cytotoxic effects on the spermatogonia cell line

(2014) Designing of a novel scaffold based on human serum albumin and hydroxyapatite nanoparticles, and the study of its cytotoxic effects on the spermatogonia cell line. Journal of Biomaterials and Tissue Engineering. pp. 638-644.

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Abstract

Here, we synthesized novel scaffolds containing human serum albumin (HSA)/hydroxyapatite nanoparticles (HA NPs) scaffold, and then their cytotoxic effects on the spermatogonial cell line (SCL) were investigated. To synthesize HA NPs, calcium nitrate and diammonium phosphate at pH 13 were gently added and heated 24 hours at 100 °C. Then serial concentrations of HA NPs was separately added to 500 mg/mL HSA, and they were straightaway held in the 100 °C water. After coagulation, all the scaffolds were cut, and incubated with SCL for 1, 2, and 3 days at 37 °C. Then, MTT assay, LDH assay, Alamar blue assay, ATP assay, vital staining, and cell counting were carried out, in order to evaluate the cytotoxicity of the scaffolds. Also, the release of calcium ions and HSA from the scaffolds was measured. The assays showed that the increase of concentration of HA NPs in the scaffolds did not change the cytotoxicity results. But, the toxicity of HSA/HA NPs scaffold on the SCL was few time-dependent. We did not observe release of calcium ions or HAS from the scaffolds at different incubation times. The new scaffold must be more investigated in vitro and in vivo, and may be suitable for artificial testes. © 2014 American Scientific Publishers

Item Type: Article
Keywords: adenosine triphosphate; hydroxyapatite nanoparticle; lactate dehydrogenase; molecular scaffold; nanoparticle; serum albumin; unclassified drug, animal cell; Article; cell counting; cell proliferation; controlled study; cytotoxicity; dynamic light scattering; energy dispersive spectroscopy; light scattering; mouse; MTT assay; nonhuman; resazurin assay; roentgen spectroscopy; scanning electron microscopy; spermatogonium; transmission electron microscopy; vital stain; X ray diffraction
Page Range: pp. 638-644
Journal or Publication Title: Journal of Biomaterials and Tissue Engineering
Volume: 4
Number: 8
Publisher: American Scientific Publishers
Depositing User: ms soheila Bazm
URI: http://eprints.ssu.ac.ir/id/eprint/9055

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