Repository of Research and Investigative Information

Repository of Research and Investigative Information

Shahid Sadoughi University of Medical Sciences

An investigation on mitochondrial DNA deletions and telomere shortening during multiple passages of adult stem cells

(2014) An investigation on mitochondrial DNA deletions and telomere shortening during multiple passages of adult stem cells. Avicenna Journal of Medical Biotechnology. pp. 156-162.

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Abstract

Background: Limited resources for adult stem cells necessitate their in vitro culture prior to clinical use. Investigating mitochondrial DNA (mtDNA) and telomere shortening has proved to be important indications of stem cell validity. This study was designed to investigate these indicators in multiple passages of three adult stem cell lines which were produced in our stem cell laboratory. Methods: In this study, Dental Pulp Stem Cells (DPSCs), Periapical Follicle Stem Cells (PAFSCs) and Human Foreskin Fibroblast (HFF) cell lines were expanded for 20 passages. After 1, 5, 10, 15 and 20 passages, expanded cells were harvested and DNA was extracted for further studies. Common mtDNA mutation was detected by multiplex PCR and telomere shortening was tested by Southern blot analysis. Results: The common deletion was not detected in any of the stem cells or cell lines after several passages. In addition, Southern blot analysis indicated that the mean difference of telomere length between first and last passage was 0.25 kb in DPSC, 0.1 kb in PAFSC and 0.32 kb in HFF which indicates that the mean telomere length in various passages of the samples showed insignificant changes. Conclusion: Absence of mtDNA mutations in adult stem cell lines indicates good mitochondrial function even after 20 passages. In addition, absence of telomere shortening indicates stem cells validity after multiple passages. It is hoped this information could pave the way for using in vitro expansion of adult stem cells for future clinical applications. © 2014, Avicenna Journal of Medical Biotechnology. All rights reserved.

Item Type: Article
Keywords: mitochondrial DNA, adolescent; adult; adult stem cell; article; cell isolation; DNA extraction; DNA sequence; fibroblast culture; gene deletion; harvesting; human; human cell; multiplex polymerase chain reaction; newborn; normal human; ovary follicle cell; polymerase chain reaction; prepuce; Southern blotting; stem cell culture; stem cell expansion; telomere shortening; thermal cycler; tooth pulp
Page Range: pp. 156-162
Journal or Publication Title: Avicenna Journal of Medical Biotechnology
Volume: 6
Number: 3
Publisher: Avicenna Research Institute
Depositing User: ms soheila Bazm
URI: http://eprints.ssu.ac.ir/id/eprint/9014

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