Repository of Research and Investigative Information

Repository of Research and Investigative Information

Shahid Sadoughi University of Medical Sciences

Designing a hydrogen peroxide biosensor using catalase and modified electrode with magne ium oxide nanoparticles

(2014) Designing a hydrogen peroxide biosensor using catalase and modified electrode with magne ium oxide nanoparticles. International Journal of Electrochemical Science. pp. 257-271.

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Abstract

In this study a combination of biology, electrochemistry and nanotechnology was used in designing a biosensor capable of detecting hydrogen peroxide (H2O2) using a modified carbon paste electrode (CPE) with magnesium oxide nanoparticles (MgO NPs) and catalase (CAT) enzyme. Synthesized MgO NPs were studied by Ultraviolet-Visible Spectroscopy (UV-VIS), X-Ray Diffraction (XRD), and Transmission Electron Microscope (TEM). All electrochemical studies were conducted using Cyclic Voltammetry (CV). Results revealed that the CAT/MgO NPs/CPE complex produced a pair of well-defined redox peaks with formal potential E° = 100 ± 2. The existence of Fe (III/II) structure in CAT molecule was an important factor that allowed the enzyme to participate in iron (Fe (III/II)) oxidation-reduction reactions; and these reactions that made it possible to design a biosensor for detecting H2O2. The designed biosensor was able to detect H2O2 in the range of 50-190 μM. also designed biosensor has a high degree of stability and retained 92 of its original activity after a given duration. ©2014 by ESG.

Item Type: Article
Page Range: pp. 257-271
Journal or Publication Title: International Journal of Electrochemical Science
Volume: 9
Number: 1
Depositing User: ms soheila Bazm
URI: http://eprints.ssu.ac.ir/id/eprint/8897

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