Abstract

To find a stable bilirubin biosensor we used of catalase and Zns nanoparticles (Nps); the detection of bilirubin in this research designed on Bioelectrochemical measurement cyclic voltammetry (CV) techniques. ZnS Nanoparticles synthesized and investigated by X-Ray diffraction (XRD), UV-Visible (UV-VIS) and scanning electron microscopy (SEM). The redox peaks studied in the range of +0.5 to -0.5 V and formal potential (Eo) calculated as 40 mv; also �Ep =70 mV. In this research the anodic peak current of showed a linear relationship for different concentrations of bilirubin in the range of 3 to 50 μM. The linear equation was expressed as ipa (μA) = -0.1417 -0.3661(μM) (R2= 0.9885) with a detection limit of 2 μM for this research. The optimum pH effect was 7.0 and maximum temperature response was around 25 °C. Interference agents not showed a significant effect on this bilirubin biosensor. © 2016 The Authors.