(2015) Preparation and Characterization of a Monoclonal Antibody Against Morphine. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. pp. 270-274.
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Abstract
A monoclonal antibody (MAb) was produced by immunization of a BALB/c mouse with a conjugated morphine C6-hemisuccinated derivative (MHS) to cationized bovine serum albumin (cBSA). The hybridoma clones were screened by indirect ELISA using MHS-BSA. The best hybridoma clone was subcloned thrice by limiting dilution. This hybridoma was found to be of IgG2b class and subclass and contained lambda light chain. The affinity of the MAb to morphine was obtained 2.8�109 M-1. The titer of the cell culture supernatant was at least 1:800. The MAb was cross-reacted with codeine (100) and apomorphine (16.5), but not with heroin, naloxone, naltrexone, or papaverine. Morphine was conjugated to HRP using a mixed anhydride method and a direct competitive ELISA was designed using anti-morphine MAb. The assay was sensitive over the 50ng/mL to 5μg/mL concentration range. In conclusion, this MAb is useful for the development of immunoassays to measure morphine in urine. © Copyright 2015, Mary Ann Liebert, Inc. 2015.
| Item Type: | Article |
|---|---|
| Keywords: | acid anhydride; apomorphine; bovine serum albumin; codeine; diamorphine; horseradish peroxidase; immunoglobulin G2b; immunoglobulin lambda chain; immunoglobulin light chain; monoclonal antibody; morphine; morphine c6 hemisuccinated derivative; morphine derivative; morphine monoclonal antibody; naloxone; naltrexone; papaverine; unclassified drug; immunoglobulin G; monoclonal antibody; morphine, animal experiment; antibody affinity; antibody specificity; Article; cell clone; cell culture; controlled study; drug cross reactivity; enzyme linked immunosorbent assay; hybridoma; immunization; immunoassay; mouse; nonhuman; priority journal; supernatant; animal; Bagg albino mouse; cross reaction; immunology; procedures, Bovinae, Animals; Antibodies, Monoclonal; Antibody Affinity; Apomorphine; Codeine; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Hybridomas; Immunization; Immunoassay; Immunoglobulin G; Mice; Mice, Inbred BALB C; Morphine |
| Page Range: | pp. 270-274 |
| Journal or Publication Title: | Monoclonal Antibodies in Immunodiagnosis and Immunotherapy |
| Volume: | 34 |
| Number: | 4 |
| Publisher: | Mary Ann Liebert Inc. |
| Depositing User: | ms soheila Bazm |
| URI: | http://eprints.ssu.ac.ir/id/eprint/9404 |
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