(2015) Magnesium oxide nanoparticles coated with glucose can silence important genes of Leishmania major at sub-toxic concentrations. Colloids and Surfaces B: Biointerfaces. pp. 300-304.
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Abstract
The aim of this study was to investigate the effect of magnesium oxide nanoparticles (MgO NPs) and MgO NPs coated with glucose (MONPCG) on Leishmania (. L) major. First, the promastigotes of L. major were separately incubated with serial concentrations of MgO NPs and MONPCG for 24, 48, and 72. h at 37. °C. Then, the cell viability of promastigotes was evaluated by MTT assay. On the other hand, the relative expression of Cpb and GP63 genes was detected by quantitative-real time PCR. Based on results, the increase of concentration, both MgO NPs and MONPCG, and incubation time led to decrease of cell viability. Moreover, the expression of Cpb and GP63 genes was decreased with increase of concentration of MgO NPs and MONPCG. Also, the increase of incubation time led to decrease of their expression in MgO NPs treated promastogotes. But, in case of MONPCG treated promastogotes, the increase of incubation time did not change the expression of Cpb and GP63. Interestingly, MONPCG could silence Cpb and GP63 genes better than MgO NPs. Note, the capability was also seen at sub-toxic concentrations of MONPCG. © 2015 Elsevier B.V.
| Item Type: | Article |
|---|---|
| Keywords: | Gene expression; Genes; Glucose; Magnesium; Nanoparticles; Polymerase chain reaction, Cell viability; Incubation time; Leishmania major; Magnesium oxide nanoparticles; MTT assays; Promastigotes; Quantitative real time PCR; Sub-toxic, Magnesia, glucose; magnesium oxide nanoparticle; nanoparticle; unclassified drug; glucose; magnesium oxide; metal nanoparticle, Article; cell viability; concentration (parameters); controlled study; Cpb gene; gene; gene expression; gene silencing; GP63 gene; incubation time; Leishmania major; MTT assay; nonhuman; physical parameters; priority journal; promastigote; quantitative analysis; real time polymerase chain reaction; chemistry; gene silencing; genetics; Leishmania major; scanning electron microscopy, Gene Silencing; Genes, Protozoan; Glucose; Leishmania major; Magnesium Oxide; Metal Nanoparticles; Microscopy, Electron, Scanning |
| Page Range: | pp. 300-304 |
| Journal or Publication Title: | Colloids and Surfaces B: Biointerfaces |
| Volume: | 136 |
| Publisher: | Elsevier |
| Depositing User: | ms soheila Bazm |
| URI: | http://eprints.ssu.ac.ir/id/eprint/9330 |
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