Abstract

Background: Abortion is one of the major complications of pregnancy. In only 50 of cases the cause of miscarriage is known. One of the most common causes of abortion is Waddlia Chondrophila (W. Chondrophila), but no information is available regarding to its presence in Iran. The purpose of this study was to develop a Taqman real-time PCR assay for the detection of W. Chondrophila in biological samples from aborted women. Methods: Clinical samples from women with a history of multiple miscarriages, normal delivery were provided. Standard strain WSU 86-1044 has been provided. The primer and probe W. Chondrophila were designed. A positive control was provided based on the PUC57 vector. Technical performance was checked using a control plasmid. The analytical sensitivity is 5 ng/mu l control plasmid DNA. Results: No cross-amplification was observed when testing for other pathogens that could be detected in human vaginitis. Taqman real-time PCR assay showed good reproducibility. This real-time PCR was then applied to 100 vaginal swabs. None of the samples tested positive for Waddlia. Conclusion: This new TaqMan real-time PCR assay represents a diagnostic tool that can be used to further investigate the prevalence of W. Chondrophila infection in patient samples.