Abstract

Objectives: MicroRNAs play a vital role in biological processes, and their irregularities have been associated with colorec-tal cancer (CRC) development. This study utilized a case-control method to investigate the possible link between two particular miRNA variations, mir-27a rs895819 and mir-423 rs6505162, and CRC susceptibility in the Iranian population. Methods: This case-control study included 120 CRC patients and 120 healthy individuals. Genotyping of the mir-27a rs895819 and mir-423 rs6505162 polymorphisms was performed using the PCR-RFLP method. The odds ratio (OR) and 95 confidence interval (95 CI) were calculated to evaluate the correlations. Results: No significant disparities were detected between the cases and controls regarding age, gender, BMI, family history of CRC, and residency. The analysis indicated that the GG genotype of the mir-27a rs895819 polymorphism was notably linked to a higher CRC risk (OR=2.134, 95 CI 1.008-4.517, p=0.048). Nevertheless, no substantial connection was noted between the mir-423 rs6505162 polymorphism and CRC susceptibility. Conclusion: The study results indicate that the mir-27a rs895819 polymorphism could serve as a potential biomarker for CRC progression. However, no correlation was found for the mir-423 rs6505162 polymorphism. Further research with diverse ethnic groups and larger sample sizes is necessary to confirm the link between these polymorphisms and CRC development. © 2024 by Eurasian Journal of Medicine and Oncology.