Abstract

Background: We aimed to investigate the molecular genotyping of Giardia duodenalis in Humans in the Yazd County, Central, Iran. Methods: Total of 35 fecal samples were collected from patients referred to Yazd Central Laboratory, Yazd, Iran from February to July 2022. All the samples were included in this study after microscopic observation of G. duodenalis. DNA samples were extracted using related kit and were ana-lyzed by Nano Drop. The molecular assessment was carried out using semi-nested PCR using the target gene of gdh. All amplified samples were sequenced using Sanger method. BLAST analyzed the sequences for as-semblage identification. Results: Out of 35 samples, 24 (68.57) and 11 (31.43) were male and female, respectively.All included samples were amplified using the specif-ic gdhprimer pair. The molecular analysis showed 17 isolates (48.57) as assemblage BIV, 8 isolates (22.86) as assemblage BIII, 6 isolates (17.14) as assemblage AII and 4 isolates (11.43) as assemblage AIII (P<0.05). Conclusion: Assemblages A and B are the most prevalent in Central Iran.The molecular identification of G. duodenalis isolates from animals and implementing control programs