Abstract

Introduction: To determine the accuracy of the assay using pANCA (perinuclear cytoplasmic antibody) and ASCA(anti saccharomyces cerevisiae antibodies) in diagnosing ulcerative colitis(UC) and crohn disease (CD).Methods: Serum samples were obtained from 62 patients with UC and11 with CD. Diagnosis was also, established by biopsy. Determination of ASCA and pANCA were performed using rapid ELISA methods and Immunometric Enzyme Immunoassay technique respectively.Results:1.6 of patients with UC against none patient with CD expressed pANCA(pv=1), 36.4 of patients with CD against 17.7 of UC patients had positive ASCA IgA (pv=0.221), 9.1 of patients with CD and 30.6of UC patients expressed positive ASCA IgG (pv=0.270) In patients with UC sensitivity, specificity, PPV and NPV of pANCA were as follows:1.61, 100, 100 and 15.27 respectively. The use of ASCA IgA test in diagnosing CD yield a sensitivity, specificity, PPV and NPV as follows: 36.36, 82.25, 26.66 and 87.93 respectively. In patients with CD the sensitivity, specificity, PPV and NPV of ASCA IgG were as follows:9.09, 69.35, 5, and 81.13 respectively.Conclusion: pANCA and ASCA testing can not be considered as a serum differential diagnosis marker for inflammatory bowel disease. © IDOSI Publications, 2012.