Abstract

In this study, the antibacterial assessment of zinc sulphide nanoparticles (ZnS NPs) against Streptococcus pyogenes and Acinetobacter baumannii is investigated. ZnS NPs were synthesized through a co-precipitation method using Polyvinylpyrrolidone (PVP), Polyvinyl alcohol (PVA), and Polyethylene Glycol (PEG). Size and morphology of the synthesized ZnS NPs are followed by a scanning electron microscope (SEM), and it is found that the size of the applied nanoparticles is about 20 nm. In order to evaluate the antibacterial effect of the synthesized ZnS NPs, various concentrations (50 µg/mL, 100 µg/mL and 150 µg/mL) were prepared. Antibacterial assessments are performed through the disc diffusion method in Mueller– Hinton agar culture medium, and the optical density (OD) method is performed by a UV-Vis spectrophotometer in Trypticase™ Soy Broth (TSB) medium. Then, in order to compare the antibacterial effects of the applied nanoparticles, several commercial antibiotics including Penicillin, Amika-cin, Ceftazidime and Primaxin antibiotics are used. The achieved results indicate that the antibacterial effect of ZnS NPs has a direct relation against the concentrations, and the concentration of 150 µg/mL shows the highest antibacterial effect in comparison with others. In addition, the nanoparticles are more effective on Acinetobacter baumannii. The findings of this research suggest a novel approach against antibacterial resistance. © 2020, G.V. Kurdyumov Institute for Metal Physics of N.A.S. of Ukraine. All rights reserved.