Abstract

In this study, a new combination drug (BH) from herbal and animal materials was designed and its anticancer activity was examined in experimental and animal models. The animal and herbal sections of drug were originated from Bee, date syrup as well as grape syrup, respectively. After drug preparation, anticancer (Human Colon Cancer (CaCO2) Cell Line), anticoagulant, and hemolysis activity were evaluated by MTT, coagulometer, and RDA methods, respectively. Two different doses of the drug were tested on rats with colon tumor in the 18-day period by determination of the size and weight of the tumor. The mice were killed after 18 days and their three important tissues (heart, kidney, and liver) were histologically evaluated. The drug has shown an effective anticancer effect on cancer cells, so that this drug completely prevents the growth of cancer cells at a low concentration within 72 hours and the percentage of live cells approximately reached zero after 72 hours. This drug has a more limited effect on normal cells with proper selectivity (Toxicity on normal cells was 32). The study of hemolysis activity on red blood cells showed that the drug had no hemolytic activity on RBCs. This drug did not effect on the coagulation system (PT and PTT pathways). The extent of the drug's ability to differentiate between cancerous and normal cells was almost constant at a time interval of 24, 48, and 72 hours. In treatment group, the weight and size of the tumor significantly decreased compared with the control group. However, there was no significant difference in comparison with the chemotherapy drug, but even in this group, the tumor size and weight reduction was higher in the new drug group. Histological data showed that both the new drug and the 5FU drug resulted in necrosis of the tumor cells. Necrosis was 75 for 5FU and 90 for the new drug. The new drug has no symptoms on the normal tissues of the body. In general, this drug can distinguish between cancerous and normal cells with a specific mechanism, and its formula is patterned from traditional medicine during extensive research. This drug can introduce as new complementary drug for cancer treatment. © 2020 Journal of Advanced Pharmacy Education & Research | Published by SPER Publication