(2016) Evaluation of AD-MSC (adipose-derived mesenchymal stem cells) as a vehicle for IFN-beta delivery in experimental autoimmune encephalomyelitis. Clinical immunology (Orlando, Fla.). pp. 98-106. ISSN 1521-7035 (Electronic) 1521-6616 (Linking)
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Abstract
Interferon-beta (IFN-beta) is commonly used as a disease modifying drug for the treatment of relapse-remitting multiple sclerosis (RR-MS). However, the underlying mechanism by which IFN-beta mediate this immunosuppressive effect is still unknown. In this study, we analyzed the effects of genetically modified adipose-derived mesenchymal stem cells (AD-MSCs) expressing murine interferon beta (MSCs-VP/IFN-beta) on the animal model of MS, experimental autoimmune encephalomyelitis (EAE). Lymph node mononuclear cells and serum were examined by using RT-PCR and ELISA methods to measure the production of IL-10 and IL-17 gene and protein expression, respectively. Our results indicated that in the MSCs-VP/IFN-beta treated group induction of Tregs and IL-10 and reduction of IL-17 were significant. Taken together, we showed that using AD-MSCs expressing IFN-beta as an anti-inflammatory agent, offer evidence supporting that the stem cell therapies in EAE conceivably will improve the valuable effects of IFN-beta in this autoimmune disease.
| Item Type: | Article |
|---|---|
| Keywords: | Adipose Tissue/*cytology Animals Blotting, Western Cells, Cultured Cytokines/genetics/immunology/metabolism Disease Models, Animal Encephalomyelitis, Autoimmune, Experimental/genetics/metabolism/*therapy Enzyme-Linked Immunosorbent Assay Female Humans Interferon-beta/genetics/*metabolism Interleukin-10/genetics/immunology/metabolism Interleukin-17/genetics/immunology/metabolism Mesenchymal Stem Cell Transplantation/*methods Mesenchymal Stem Cells/cytology/*metabolism Mice Mice, Inbred C57BL Multiple Sclerosis/genetics/metabolism/therapy Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes, Regulatory/immunology/metabolism Eae IFN-beta MSCs (mesenchymal stem cells) Treg |
| Page Range: | pp. 98-106 |
| Journal or Publication Title: | Clinical immunology (Orlando, Fla.) |
| Volume: | 169 |
| Identification Number: | https://doi.org/10.1016/j.clim.2016.06.015 |
| ISSN: | 1521-7035 (Electronic) 1521-6616 (Linking) |
| Depositing User: | Mr mahdi sharifi |
| URI: | http://eprints.ssu.ac.ir/id/eprint/31322 |
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