(2022) L-proline as a novel additive to cryopreservation media improved post-thaw quality of human spermatozoon via reducing oxidative stress. Andrologia.
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Abstract
Sperm cryopreservation as a routine technique in assisted reproductive technique (ART) laboratories has detrimental effects on spermatozoa. Various methods have been introduced to improve it. The aim of this research was to evaluate the effects of L-proline supplementation in cryopreservation medium on normozoospermic semen samples. A total of 30 semen samples were collected from normozoospermic men. Cryopreservation media were supplemented with different concentrations of L-proline (0, 1, 2 and 4 mmol/L). The semen samples were cryopreserved. After thawing, sperm parameters and chromatin integrity (aniline blue (AB), toluidine blue (TB), sperm chromatin dispersion test (SCD) and chromomycin A3 (CMA3)), reactive oxygen species (ROS), and total antioxidant capacity (TAC) and malondialdehyde (MDA) levels were evaluated. A total of 4 mmol/L L-proline significantly improved progressive motility and viability (p < 0.05). MDA and ROS levels significantly diminished in samples were cryopreserved by 4 mmol/L L-proline supplemented cryopreservation media (p < 0.001). Also, it significantly increased TAC level. Also, chromatin damages (AB, TB and CMA3) significantly improved in samples were cryopreserved by 4 mmol/L L-proline supplemented cryopreservation media (p < 0.05). The results support that the usage of L-proline supplemented cryopreservation media to improve sperm quality after cryopreservation. © 2021 Wiley-VCH GmbH
| Item Type: | Article |
|---|---|
| Keywords: | 2'7' dichlorofluorescein diacetate; antioxidant; chromomycin A3; cryoprotective agent; dichlorofluorescein; drug additive; liquid nitrogen; malonaldehyde; proline; reactive oxygen metabolite; tolonium chloride; trolox C; antioxidant; proline, adult; Article; chromatin; clinical article; controlled study; cryopreservation; enzyme linked immunosorbent assay; human; human cell; human tissue; male; masturbation; morphology; oxidative stress; semen cryopreservation; semen parameters; sexual abstinence; sperm count; sperm preparation; sperm viability; spermatozoon; spermatozoon motility; staining; supplementation; thawing; World Health Organization; cryopreservation; metabolism; oxidative stress; semen analysis; sperm preservation; spermatozoon, Antioxidants; Cryopreservation; Cryoprotective Agents; Humans; Male; Oxidative Stress; Proline; Semen Analysis; Semen Preservation; Sperm Motility; Spermatozoa |
| Journal or Publication Title: | Andrologia |
| Volume: | 54 |
| Number: | 1 |
| Depositing User: | ms soheila Bazm |
| URI: | http://eprints.ssu.ac.ir/id/eprint/12818 |
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