Repository of Research and Investigative Information

Repository of Research and Investigative Information

Shahid Sadoughi University of Medical Sciences

ANXA2, SP17, SERPINA5, PRDX2 genes, and sperm DNA fragmentation differentially represented in male partners of infertile couples with normal and abnormal sperm parameters

(2022) ANXA2, SP17, SERPINA5, PRDX2 genes, and sperm DNA fragmentation differentially represented in male partners of infertile couples with normal and abnormal sperm parameters. Andrologia.

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

This study aims to evaluate the expression of genes associated with the fertilisation potential and embryo development, sperm DNA fragmentation (SDF), and acrosome reaction in male partners of infertile couples with different sperm parameters compared to fertile men. First, male partners of infertile couples with abnormal (N = 25) and normal sperm parameters (N = 25), and fertile men (N = 10) were included in experimental groups I, II, and controls respectively. The mRNA levels of the Annexin A2 (ANXA2), Sperm protein 17 (SP17), Plasma serine protease inhibitor (SERPINA5), and Peroxiredoxin-2 (PRDX2) genes and SDF were evaluated. To evaluate the maturity of the sperm and oxidative stress, the acrosome reaction, the lipid peroxidation, and total antioxidant were measured. As result, SP17 showed a significantly lower expression in both experimental groups. SERPINA5 was significantly down-regulated in experimental group I that was aligned with the low rate of acrosome reaction. Significant overexpression of PRDX2 was found between experimental group II and controls. Significant higher rates of SDF were seen in both experimental groups compared to the controls. Finally, our data suggest that differentially gene expression of SP17 is a potential diagnostic biomarker in infertile men either with normal or abnormal sperm parameters. SDF is one of the causes of male infertility, independent of the sperm parameters. © 2022 Wiley-VCH GmbH.

Item Type: Article
Keywords: acridine orange; agarose; beta 2 microglobulin; bleaching agent; cacodylic acid; chromomycin A3; distilled water; double stranded DNA; glutaraldehyde; glyceraldehyde 3 phosphate dehydrogenase; hydrochloric acid; lipocortin 2; malonaldehyde; membrane protein; messenger RNA; peroxiredoxin 2; protamine; rose bengal; serine proteinase inhibitor; single stranded DNA; sperm surface protein 17; unclassified drug; antioxidant; ANXA2 protein, human; biological marker; lipocortin 2; messenger RNA; peroxiredoxin; plasminogen activator inhibitor 3; PRDX2 protein, human; serine proteinase inhibitor; SERPINA5 protein, human, acrosome reaction; adult; Article; controlled study; cross-sectional study; DNA fragmentation; down regulation; embryo development; fertilization; gene expression; gene expression level; gene overexpression; gene sequence; genetic association; human; lipid peroxidation; major clinical study; male; male fertility; male infertility; oxidative stress; semen analysis; semen parameters; sperm; sperm count; spermatozoon density; spermatozoon maturation; spermatozoon motility; total antioxidant capacity; DNA fragmentation; genetics; male infertility; metabolism; sperm; spermatozoon, Annexin A2; Antioxidants; Biomarkers; DNA Fragmentation; Humans; Infertility, Male; Male; Peroxiredoxins; Protein C Inhibitor; RNA, Messenger; Semen; Serine Proteinase Inhibitors; Spermatozoa
Journal or Publication Title: Andrologia
Volume: 54
Number: 10
Publisher: John Wiley and Sons Inc
Depositing User: ms soheila Bazm
URI: http://eprints.ssu.ac.ir/id/eprint/12284

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