(2021) Sperm and testicular dysfunction during cecal ligation and puncture-induced sepsis in male rats and effects of tannic acid through reducing testicular oxidative stress and inflammation. Iranian Journal of Basic Medical Sciences. pp. 1554-1560.
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Abstract
Objective(s): One of the problems caused by infectious diseases is the decrease in sperm count and motility. Tannic acid is known as an anti-oxidant and anti-inflammatory agent. In this study, Cecal Ligation and Puncture (CLP) sepsis model was induced to investigate the effect of tannic acid on oxidative stress and inflammation in testicular and sperm structure and function. Materials and Methods: Twenty-four male Wistar rats (250-300 g) were randomly divided into 3 groups of 8: 1) sham, 2) sepsis, and 3) sepsis + tannic acid (20 mg/kg at 6, 12, and 24 hr after sepsis induction). Thirty hours after induction of sepsis, testicular samples were collected to measure SOD activity and MDA, IL-6, and TNF-α levels. Another part of the testis was fixed in 10 formalin for histological examinations. Results: In the sepsis group, testicular MDA, TNF-α, and IL-6 levels increased and SOD activity decreased compared with the sham group. In addition, the percentage of motile sperm and the survival rate of sperm decreased significantly in the sepsis group. Administration of tannic acid significantly decreased inflammatory markers (TNF-α and IL-6) and MDA levels and increased SOD activity. Furthermore tannic acid significantly improved sperm parameters and increased sperm and animal survival rates. Conclusion: The results of this study showed that the reproductive system may be strongly affected by the conditions created during sepsis. Tannic acid improved reproductive dysfunction in sepsis by reducing oxidative stress and inflammation. © 2021 Mashhad University of Medical Sciences. All rights reserved.
| Item Type: | Article |
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| Keywords: | actin antibody; alcohol; antiinflammatory agent; antioxidant; bovine serum albumin; buffer; deionized water; distilled water; edetic acid; eosin; formaldehyde; hematoxylin; ice; interleukin 6; ketamine; ketorolac; malonaldehyde; paraffin; protein Bax; protein bcl 2; proteinase inhibitor; sodium chloride; superoxide dismutase; tannin; tetracycline; thiobarbituric acid; triton x 100; tumor necrosis factor; water; xylazine, abdominal cavity; abdominal viscera; anesthesia; animal cell; animal experiment; animal model; animal tissue; antiinflammatory activity; antioxidant activity; arterial oxygen tension; Article; autoradiography; blood sampling; blood vessel; body temperature; cecal ligation and puncture-induced sepsis; cecum; cell lineage; centrifugation; chemoluminescence; connective tissue; controlled study; densitometry; ear swab; electrochemiluminescence; enzyme activity; enzyme linked immunosorbent assay; epididymis; fluid resuscitation; genital system; germ cell; histology; immunoreactivity; incubation time; inflammation; interstitium; laparotomy; light dark cycle; male; microscopy; microtiter plate assay; nonhuman; oxidative stress; paraffin embedding; peritoneal cavity; polyacrylamide gel electrophoresis; post hoc analysis; pulse oximetry; randomized controlled trial; rat; seminiferous tubule; sepsis; Sertoli cell; small intestine; sperm; sperm count; sperm function; sperm viability; spermatogonium; spermatozoon motility; systolic blood pressure; testis injury; testis tissue; tissue degeneration; tissue section; total motile sperm count; ultrasound; Western blotting; wrinkle |
| Page Range: | pp. 1554-1560 |
| Journal or Publication Title: | Iranian Journal of Basic Medical Sciences |
| Volume: | 24 |
| Number: | 11 |
| Depositing User: | ms soheila Bazm |
| URI: | http://eprints.ssu.ac.ir/id/eprint/12073 |
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