Abstract

Background is an opportunistic pathogen that could be resistant to many antimicrobial agents. Resistance genes can be carried among gram-negative bacteria by integrons. Enzymatic inactivation is the most important mechanism of resistance to aminoglycosides. In this study, the frequencies of two important resistance gene a and and genes coding integrase I and II, in isolates resistant to aminoglycosides were evaluated. Methods In this cross-sectional study, an attempt was made to assess the antibiotic susceptibility of 130 isolates obtained from different samples of patients hospitalized in training hospitals of Yazd evaluated by disk diffusion method. The frequencies of a, , and genes were determined by PCR method. Data were analyzed by chi-square method using SPSS software (Ver. 16). Results our results showed that resistance to gentamicin, tobramycin, kanamycin, and amikacin were 34.6, 33.8, 43.8, and 14.6%, respectively. The frequencies of a, , and genes were 44.6, 27.7, 90, and 0%, respectively. Conclusion This study showed there are high frequencies of genes coding aminoglycosides resistance in isolates. Hence, it is very important to monitor and inhibit the spread of antibiotic resistance genes.